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bright field illumination  (Nikon)


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    Structured Review

    Nikon bright field illumination
    Bright Field Illumination, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 591 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/illumination+field/pmc12884168-109-6-10?v=Nikon
    Average 96 stars, based on 591 article reviews
    bright field illumination - by Bioz Stars, 2026-07
    96/100 stars

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    (A) Schematic overview of FOLIC, a bioinspired hybrid camera that employs a multi-aperture concave architecture that integrates the modular ommatidial layout characteristics of arthropod compound eyes with the high acuity, on-axis focal properties of vertebrate chambered eyes. (B) Framework of FOLIC, illustrating elemental image formation and reconstruction. The upper-right inset shows the EDOF produced by the logarithmic lenslets. The FOLIC images feature three distinct functional zones: peripheral, blend, and foveated, each uniquely contributing <t>to</t> <t>wide-field</t> context, scalable depth perception, and high-resolution volumetric reconstruction, respectively. The illustration was generated using Adobe Illustrator and Autodesk Fusion.
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    Image Search Results


    (A) Schematic overview of FOLIC, a bioinspired hybrid camera that employs a multi-aperture concave architecture that integrates the modular ommatidial layout characteristics of arthropod compound eyes with the high acuity, on-axis focal properties of vertebrate chambered eyes. (B) Framework of FOLIC, illustrating elemental image formation and reconstruction. The upper-right inset shows the EDOF produced by the logarithmic lenslets. The FOLIC images feature three distinct functional zones: peripheral, blend, and foveated, each uniquely contributing to wide-field context, scalable depth perception, and high-resolution volumetric reconstruction, respectively. The illustration was generated using Adobe Illustrator and Autodesk Fusion.

    Journal: bioRxiv

    Article Title: Foveated Light-Field Compound Imager

    doi: 10.64898/2026.03.23.713670

    Figure Lengend Snippet: (A) Schematic overview of FOLIC, a bioinspired hybrid camera that employs a multi-aperture concave architecture that integrates the modular ommatidial layout characteristics of arthropod compound eyes with the high acuity, on-axis focal properties of vertebrate chambered eyes. (B) Framework of FOLIC, illustrating elemental image formation and reconstruction. The upper-right inset shows the EDOF produced by the logarithmic lenslets. The FOLIC images feature three distinct functional zones: peripheral, blend, and foveated, each uniquely contributing to wide-field context, scalable depth perception, and high-resolution volumetric reconstruction, respectively. The illustration was generated using Adobe Illustrator and Autodesk Fusion.

    Article Snippet: A blue LED (M470L3, Thorlabs) was roughly collimated to provide wide-field illumination of the sample.

    Techniques: Produced, Functional Assay, Generated

    (A-C) Elemental (A), reconstructed FOLIC (B), and wide-field ground-truth (C) images of 15-µm fluorescent microspheres distributed in three layers. The dashed box in (A) outlines the sensor area. The arrows point to exemplary consistent features. (D-F) Reconstructed focal images by FOLIC at Δ Z 1 = 708 µm, Δ Z 2 = 837 µm, and Δ Z 3 = 963 µm, where peripheral, blend, and foveated zones were outlined. (G-I) Corresponding wide-field images at each layer, respectively. The arrows point to exemplary consistent features. Microsphere structures as narrow as ∼14 µm were consistently resolved in (F) and (I). (J) Elemental image of the central lenslet, which was employed as a mask to suppress noise and reconstruction artifacts. The green arrow indicates a pre-scaling feature displacement. (K) Corresponding wide-field images projected with 1250 axial stacks (step size = 2 µm). (L, M) Focal images of live HeLa cells embedded within a 2-mm-thick hydrogel, reconstructed by FOLIC at Δ Z 1 = 192 µm, Δ Z 2 = 966 µm. Peripheral, blend, and foveated zones were outlined. (N, O) Corresponding wide-field images at each layer, respectively. The arrows point to exemplary consistent features. (P, Q) Wide-field volume (P) and the corresponding reconstructed volume captured by FOLIC (Q), with clearly indicated foveated (red cones), blend (blue cones), and peripheral zones (single-layer view). Scale bars: 200 µm.

    Journal: bioRxiv

    Article Title: Foveated Light-Field Compound Imager

    doi: 10.64898/2026.03.23.713670

    Figure Lengend Snippet: (A-C) Elemental (A), reconstructed FOLIC (B), and wide-field ground-truth (C) images of 15-µm fluorescent microspheres distributed in three layers. The dashed box in (A) outlines the sensor area. The arrows point to exemplary consistent features. (D-F) Reconstructed focal images by FOLIC at Δ Z 1 = 708 µm, Δ Z 2 = 837 µm, and Δ Z 3 = 963 µm, where peripheral, blend, and foveated zones were outlined. (G-I) Corresponding wide-field images at each layer, respectively. The arrows point to exemplary consistent features. Microsphere structures as narrow as ∼14 µm were consistently resolved in (F) and (I). (J) Elemental image of the central lenslet, which was employed as a mask to suppress noise and reconstruction artifacts. The green arrow indicates a pre-scaling feature displacement. (K) Corresponding wide-field images projected with 1250 axial stacks (step size = 2 µm). (L, M) Focal images of live HeLa cells embedded within a 2-mm-thick hydrogel, reconstructed by FOLIC at Δ Z 1 = 192 µm, Δ Z 2 = 966 µm. Peripheral, blend, and foveated zones were outlined. (N, O) Corresponding wide-field images at each layer, respectively. The arrows point to exemplary consistent features. (P, Q) Wide-field volume (P) and the corresponding reconstructed volume captured by FOLIC (Q), with clearly indicated foveated (red cones), blend (blue cones), and peripheral zones (single-layer view). Scale bars: 200 µm.

    Article Snippet: A blue LED (M470L3, Thorlabs) was roughly collimated to provide wide-field illumination of the sample.

    Techniques:

    ( A - C ) Elemental (A), reconstructed FOLIC (B), and wide-field (C) images of a 15-μm-thick mouse kidney section labeled with WGA, featuring glycoprotein-rich regions (e.g., distal tubules). Peripheral, blend, and foveated zones were delineated. The effect of contrast adjustment was shown relative to the raw reconstruction in (B). ( D, E ) Zoomed-in images of the regions in (B, C) as marked, displaying enhanced clarity and resolution in the foveated regions compared to the blend regions. ( F, G ) Cross-sectional profiles along the dashed lines as marked in (D, E). ( H ) Brightfield elemental images of Hymenoptera ant specimens using fiber-based white-light illumination. ( I ) Zoomed-in image of the elemental image of the central lenslet in (H). ( J, K ) Reconstructed FOLIC images at Δ Z 1 = 0 µm and Δ Z 2 = 111 µm with contrast adjustment. Arrows point to features in focus. ( L ) Brightfield elemental images of Hymenoptera ant samples using cell-phone flashlight illumination. ( M, N ) Reconstructed focal images by FOLIC at Δ Z 1 = 0 µm and Δ Z 2 = 168 µm, respectively. ( O, P ) Cross-sectional profiles of the structures as marked in (M, N) at the two focal planes. ( Q ) Volumetric view of the reconstructed ant specimen containing all focal layers under ambient illumination. Scale bars: 200 µm.

    Journal: bioRxiv

    Article Title: Foveated Light-Field Compound Imager

    doi: 10.64898/2026.03.23.713670

    Figure Lengend Snippet: ( A - C ) Elemental (A), reconstructed FOLIC (B), and wide-field (C) images of a 15-μm-thick mouse kidney section labeled with WGA, featuring glycoprotein-rich regions (e.g., distal tubules). Peripheral, blend, and foveated zones were delineated. The effect of contrast adjustment was shown relative to the raw reconstruction in (B). ( D, E ) Zoomed-in images of the regions in (B, C) as marked, displaying enhanced clarity and resolution in the foveated regions compared to the blend regions. ( F, G ) Cross-sectional profiles along the dashed lines as marked in (D, E). ( H ) Brightfield elemental images of Hymenoptera ant specimens using fiber-based white-light illumination. ( I ) Zoomed-in image of the elemental image of the central lenslet in (H). ( J, K ) Reconstructed FOLIC images at Δ Z 1 = 0 µm and Δ Z 2 = 111 µm with contrast adjustment. Arrows point to features in focus. ( L ) Brightfield elemental images of Hymenoptera ant samples using cell-phone flashlight illumination. ( M, N ) Reconstructed focal images by FOLIC at Δ Z 1 = 0 µm and Δ Z 2 = 168 µm, respectively. ( O, P ) Cross-sectional profiles of the structures as marked in (M, N) at the two focal planes. ( Q ) Volumetric view of the reconstructed ant specimen containing all focal layers under ambient illumination. Scale bars: 200 µm.

    Article Snippet: A blue LED (M470L3, Thorlabs) was roughly collimated to provide wide-field illumination of the sample.

    Techniques: Labeling